Conventional optothermal microbubble assisted bio-sensing is not suitable for protein sensing due to the high temperatures over 100 dgrees C. To overcome this problem, researchers have demonstrated a proof-of-concept study that reduces the working temperature of bubble generation simply by introducing a volatile, water-immiscible liquid into an aqueous medium. This enables the generation of bubbles at a much lower temperature of around 30 degrees C, not only making this sensing platform compatible with proteins but also enhancing the surface capture of proteins.