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Hi E, check out this Fierce Whitepaper we think you'll like:

 

Considerations and Guidelines for Real-Time qPCR Optimization

 

  
 

 

Optimizing qPCR can seem overwhelming and requires careful experimental design that evaluates several performance factors for each reagent formulation tested. This White Paper discusses the different approaches to real-time qPCR, outlines considerations to help quickly compare previously optimized qPCR assays with a new qPCR Master Mix, offers guidance for optimizing your real-time qPCR assay and provides a tool for comparing qPCR reagent formulations.

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